The C. elegans RSA Complex Localizes Protein Phosphatase 2A to Centrosomes and Regulates Mitotic Spindle Assembly

نویسندگان

  • Anne-Lore Schlaitz
  • Martin Srayko
  • Alexander Dammermann
  • Sophie Quintin
  • Natalie Wielsch
  • Ian MacLeod
  • Quentin de Robillard
  • Andrea Zinke
  • John R. Yates
  • Thomas Müller-Reichert
  • Andrei Shevchenko
  • Karen Oegema
  • Anthony A. Hyman
چکیده

Microtubule behavior changes during the cell cycle and during spindle assembly. However, it remains unclear how these changes are regulated and coordinated. We describe a complex that targets the Protein Phosphatase 2A holoenzyme (PP2A) to centrosomes in C. elegans embryos. This complex includes Regulator of Spindle Assembly 1 (RSA-1), a targeting subunit for PP2A, and RSA-2, a protein that binds and recruits RSA-1 to centrosomes. In contrast to the multiple functions of the PP2A catalytic subunit, RSA-1 and RSA-2 are specifically required for microtubule outgrowth from centrosomes and for spindle assembly. The centrosomally localized RSA-PP2A complex mediates these functions in part by regulating two critical mitotic effectors: the microtubule destabilizer KLP-7 and the C. elegans regulator of spindle assembly TPXL-1. By regulating a subset of PP2A functions at the centrosome, the RSA complex could therefore provide a means of coordinating microtubule outgrowth from centrosomes and kinetochore microtubule stability during mitotic spindle assembly.

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عنوان ژورنال:
  • Cell

دوره 128  شماره 

صفحات  -

تاریخ انتشار 2007